Publications

This comprehensive review examines the current state of menstrual blood-derived mesenchymal stem cell (MenSC) research, comparing isolation methodologies, characterizing phenotypic markers, and evaluating preclinical therapeutic applications. We present standardized protocols for collection, isolation, and culture that optimize cell yield while maintaining ISCT-compliant characterization standards.

A head-to-head comparison of Ficoll-Paque density gradient centrifugation versus ammonium chloride RBC lysis for isolating menstrual blood-derived stem cells. We evaluate yield, viability, purity, and downstream culture characteristics to provide evidence-based recommendations for protocol selection.

The International Society for Cellular Therapy (ISCT) position statement establishing minimum criteria for defining human mesenchymal stem cells: plastic adherence, specific surface antigen expression, and trilineage differentiation potential.

Landmark study demonstrating that menstrual blood contains stromal stem cells with multipotent differentiation capacity, capable of differentiating into chondrogenic, adipogenic, and neurogenic lineages.

Original characterization of endometrial regenerative cells (ERCs) from menstrual blood, demonstrating unique immunological properties and expansion capacity exceeding 68 doublings.

Demonstration that MenSCs can differentiate into cardiac precursor cells with potential applications in cardiovascular regenerative medicine.

Standardized protocols for menstrual blood sample collection, including donor screening, collection device preparation, transport media specifications, and chain of custody documentation.

Detailed procedures for isolating mesenchymal stem cells from menstrual blood using density gradient centrifugation and RBC lysis methods, including troubleshooting and quality control checkpoints.

Complete culture protocols including media formulations, seeding densities, feeding schedules, passaging criteria, and cryopreservation procedures for MenSC maintenance and expansion.

ISCT-compliant flow cytometry panel for MenSC characterization, including surface marker selection, gating strategies, and quality control thresholds for positive and negative marker expression.

Standardized protocols for osteogenic, adipogenic, and chondrogenic differentiation of MenSCs, including induction media formulations, staining procedures, and validation criteria.

Multiple methods for extracellular vesicle isolation from MenSC conditioned media, including ultracentrifugation, precipitation, and size exclusion chromatography approaches.